PAB19961

antibody from Abnova Corporation

Targeting: TPM1 C15orf13, CMH3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB19961

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB19961, RRID:AB_10962880

Product name

TPM1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant TPM1.

Antigen sequence

KVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKY
EEVARKLVIIESDLERAEERAELSEGQVRQLEEQL
RIMDQTLKALMAAEDKYSQKEDRYEEEIKVLSDKL
KEAETRAEFAE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with TPM1 polyclonal antibody (Cat # PAB19961).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with TPM1 polyclonal antibody (Cat # PAB19961) at 1-4 ug/mL dilution shows positivity in cytoplasm, cytoskeleton.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human smooth muscle with TPM1 polyclonal antibody (Cat # PAB19961) shows moderate cytoplasmic positivity in smooth muscle cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)