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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [2]
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- Product number
- GTX121246 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121246, RRID:AB_10722382
- Product name
- KDM6A antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references UTX-mediated enhancer and chromatin remodeling suppresses myeloid leukemogenesis through noncatalytic inverse regulation of ETS and GATA programs.
Lkb1 inactivation drives lung cancer lineage switching governed by Polycomb Repressive Complex 2.
Gozdecka M, Meduri E, Mazan M, Tzelepis K, Dudek M, Knights AJ, Pardo M, Yu L, Choudhary JS, Metzakopian E, Iyer V, Yun H, Park N, Varela I, Bautista R, Collord G, Dovey O, Garyfallos DA, De Braekeleer E, Kondo S, Cooper J, Göttgens B, Bullinger L, Northcott PA, Adams D, Vassiliou GS, Huntly BJP
Nature genetics 2018 Jun;50(6):883-894
Nature genetics 2018 Jun;50(6):883-894
Lkb1 inactivation drives lung cancer lineage switching governed by Polycomb Repressive Complex 2.
Zhang H, Fillmore Brainson C, Koyama S, Redig AJ, Chen T, Li S, Gupta M, Garcia-de-Alba C, Paschini M, Herter-Sprie GS, Lu G, Zhang X, Marsh BP, Tuminello SJ, Xu C, Chen Z, Wang X, Akbay EA, Zheng M, Palakurthi S, Sholl LM, Rustgi AK, Kwiatkowski DJ, Diehl JA, Bass AJ, Sharpless NE, Dranoff G, Hammerman PS, Ji H, Bardeesy N, Saur D, Watanabe H, Kim CF, Wong KK
Nature communications 2017 Apr 7;8:14922
Nature communications 2017 Apr 7;8:14922
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: IMR32 5% SDS PAGE GTX121246 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- KDM6A antibody [N2C1], Internal detects KDM6A protein by western blot analysis.A. 30 ?g U87-MG whole cell lysate/extract B. 30 ?g SK-N-SH whole cell lysate/extract C. 30 ?g IMR32 whole cell lysate/extract D. 30 ?g SK-N-AS whole cell lysate/extract5% SDS-PAGEKDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(GTX121246) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments.IP samples: SK-N-SH whole cell extractA. 30 £gg SK-N-SH whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of KDM6A protein by 4 £gg KDM6A antibody [N2C1], Internal (GTX121246)5 % SDS-PAGEThe immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(GTX121246) antibody at 1:250 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with HeLa chromatin extract and 5 ?g of either control rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.
- Validation comment
- ChIP
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with HeLa chromatin extract and 5 £gg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.
