Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
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- Product number
- MA5-30722 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OBP2B Recombinant Rabbit Monoclonal Antibody (002)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire. This antibody has specificity for Human OBP2B.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B.
Sano R, Takahashi Y, Fukuda H, Harada M, Hayakawa A, Okawa T, Kubo R, Takeshita H, Tsukada J, Kominato Y
Scientific reports 2021 Apr 1;11(1):7325
Scientific reports 2021 Apr 1;11(1):7325
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 3 Quantitative decrease of OBP2B protein in the mutant clones B3 and B4 relative to that in the wild-type KATOIII cells. The amount of OBP2B or beta-actin protein was evaluated by Western blotting using the cell lysate and supernatant prepared from the wild-type KATOIII cells and its derived mutant clones B3 and B4, followed by densitometry measurements. The representative blots are shown in the upper panels. The two panels on the left side show the blots of OBP2B obtained using a cell lysate after short or long exposure. The molecular weight of each protein was estimated using ECL DualVue Western blotting markers (cytiva). The amount of protein applied to each lane was 30 mug for the cell lysate or 20 mug for the supernatant. The left and right lower panels indicate the relative amounts of OBP2B protein in the cell lysate and supernatant, respectively, obtained from the mutant cells when that of the wild-type cells was assigned an arbitrary value of 1.0. The middle lower panel indicates the level of OBP2B normalized to the amount of beta-actin in cell lysates in the mutant cells, relative to that for wild-type cells which was assigned an arbitrary value of 1.0. The relative level of OBP2B represents the mean from more than three independent experiments. The significance of the decrease was determined by Student''s t test at a significance level of p < 0.01 (**).