HPA045679

antibody from Atlas Antibodies

Targeting: HDGFL1 dJ309H15.1, Hdgfrp1, HRP-1, PWWP1

Antibody data

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Validation data

Product number: HPA045679 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA045679, RRID:AB_2679415
Product name: Anti-HDGFL1
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: ELREEEVADEEASQEWHAEAPGGGDRDS
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

HDGFL1 protein structure - HPA045679 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human testis and endometrium tissues using Anti-HDGFL1 antibody. Corresponding HDGFL1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Immunohistochemical staining of human colon, kidney, liver and testis using Anti-HDGFL1 antibody HPA045679 (A) shows similar protein distribution across tissues to independent antibody HPA063707 (B).
Antibody #2 product nr: HPA063707
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferus ducts.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human endometrium shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-HDGFL1 antibody HPA045679.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-HDGFL1 antibody HPA045679.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-HDGFL1 antibody HPA045679.
Sample type: HUMAN