12263-1-AP

antibody from Invitrogen Antibodies

Targeting: CCT8 C21orf112, Cctq, PRED71

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12263-1-AP

Provider

Invitrogen Antibodies

Product name

CCT8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: FNVDNIRVC KILGSGISSS SVLHGMVFKK ETEGDVTSVK DAKIAVYSCP FDGMITETKG TVLIKTAEEL MNFSKGEENL MDAQVKAIAD TGANVVVTGG KVADMALHYA NKYNIMLVRL NSKWDLRRLC KTVGATALPR LTPPVLEEMG HCDSVYLSEV GDTQVVVFKH EKEDGAISTI VLRGSTDNLM DDIERAVDDG VNTFKVLTRD KRLVPGGGAT EIELAKQITS YGETCPGLEQ YAIKKFAEAF EAIPRALAEN SGVKANEVIS KLYAVHQEGN KNVGLDIEAE VPAVKDMLEA GILDTYLGKY WAIKLATNAA VTVLRVDQII MAKPAGGPKP PSGKKDWDDD QND (146-497 aa encoded by BC012584)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Raji cells were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12263-1-AP ( CCT8 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 12263-1-AP (CCT8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CCT8 (IP:12263-1-AP, 4ug; Detection:12263-1-AP 1:500) with Jurkat cells lysate 3160 ug.