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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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Validation data
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- Product number
- 20500-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF557 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: CRNLASLGN QVDKPRLISQ LEQEDKVMTE ERGILSGTCP DVENPFKAKG LTPKLHVFRK EQSRNMKMER NHLGATLNEC NQCFKVFSTK SSLTRHRKIH TGERPYGCSE CGKSYSSRSY LAVHKRIHNG EKPYECNDCG KTFSSRSYLT VHKRIHNGEK PYECSDCGKT FSNSSYLRPH LRIHTGEKPY KCNQCFREFR TQSIFTRHKR VHTGEGHYVC NQCGKAFGTR SSLSSHYSIH TGEYPYECHD CGRTFRRRSN LTQHIRTHTG EKPYTCNECG KSFTNSFSLT IHRRIHNGEK S (76-376 aa encoded by BC003020)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.36 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP ( ZNF557 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 20500-1-AP (ZNF557 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 20500-1-AP (ZNF557 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 20500-1-AP (ZNF557 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
