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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF395 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Ephrin-B3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Ephrin-B3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Ephrin-A5 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization.
Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors.
Yang NY, Pasquale EB, Owen LB, Ethell IM
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
The Journal of biological chemistry 2006 Oct 27;281(43):32574-86
Intracellular and trans-synaptic regulation of glutamatergic synaptogenesis by EphB receptors.
Kayser MS, McClelland AC, Hughes EG, Dalva MB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 22;26(47):12152-64
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Nov 22;26(47):12152-64
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse and Rat Ephrin-B3 by Western Blot. Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Ephrin-B3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF395) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Ephrin-B3 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
