PAB10969

antibody from Abnova Corporation

Targeting: FOXO1 FKH1, FKHR, FOXO1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10969

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10969, RRID:AB_1708012

Product name

FOXO1 (phospho S256) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Hepatic insulin resistance in ob/ob mice involves increases in ceramide, aPKC activity, and selective impairment of Akt-dependent FoxO1 phosphorylation.

Sajan MP, Ivey RA, Lee MC, Farese RV
Journal of lipid research 2015 Jan;56(1):70-80

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PKCλ haploinsufficiency prevents diabetes by a mechanism involving alterations in hepatic enzymes.

Sajan MP, Ivey RA 3rd, Lee M, Mastorides S, Jurczak MJ, Samuels VT, Shulman GI, Braun U, Leitges M, Farese RV
Molecular endocrinology (Baltimore, Md.) 2014 Jul;28(7):1097-107

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of extracts from 293 cells untreated (Lane 1) or treated (Lane 2) with serum using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence staining of manthanol-fixed MCF-7 cells using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embbeded human breast carcinoma tissue using FOXO1 (phospho S256) polyclonal antibody (Cat # PAB10969) (A) or preincubated with blocking peptide (B).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)