LS-C801166

antibody from LSBio

Targeting: FOXO1 FKH1, FKHR, FOXO1A

Western blot

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: LS-C801166 - Provider product page
Provider: LSBio
Product name: FOXO1 / FKHR Antibody (phospho-Ser319) LS-C801166
Antibody type: Polyclonal
Description: Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Storage: Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

FOXO1 protein structure - LS-C801166 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:50 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: 1:200 staining human colon carcinoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.