Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [2]
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Validation data
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- Product number
- GTX84331 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84331, RRID:AB_10726712
- Product name
- Grp75 antibody [9F8]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA9 monoclonal antibody.
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- GeneTex (provider)
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- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Anti-HSPA9 mouse monoclonal antibody (GTX84331) immunofluorescent staining of COS7 cells transiently transfected with HSPA9
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody (GTX84331).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using antiHSPA9 mouse monoclonal antibody. (GTX84331, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using antiHSPA9mouse monoclonal antibody. (GTX84331, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using antiHSPA9mouse monoclonal antibody. (GTX84331, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue using antiHSPA9mouse monoclonal antibody. (GTX84331, Dilution 1:50)
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- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using antiHSPA9mouse monoclonal antibody. (GTX84331, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-HSPA9 antibody (GTX84331), (Red), compared to a nonspecific negative control antibody, (Blue).
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- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-HSPA9 antibody (GTX84331), (Red), compared to a nonspecific negative control antibody, (Blue).