Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX104362 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104362, RRID:AB_11171955
- Product name
- GLI3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Blockade of Hedgehog Signaling Attenuates Biliary Cystogenesis in the Polycystic Kidney (PCK) Rat.
Hedgehog signaling is a potent regulator of liver lipid metabolism and reveals a GLI-code associated with steatosis.
Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels.
Sato Y, Yamamura M, Sasaki M, Harada K
The American journal of pathology 2018 Oct;188(10):2251-2263
The American journal of pathology 2018 Oct;188(10):2251-2263
Hedgehog signaling is a potent regulator of liver lipid metabolism and reveals a GLI-code associated with steatosis.
Matz-Soja M, Rennert C, Schönefeld K, Aleithe S, Boettger J, Schmidt-Heck W, Weiss TS, Hovhannisyan A, Zellmer S, Klöting N, Schulz A, Kratzsch J, Guthke R, Gebhardt R
eLife 2016 May 17;5
eLife 2016 May 17;5
Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels.
Matz-Soja M, Aleithe S, Marbach E, Böttger J, Arnold K, Schmidt-Heck W, Kratzsch J, Gebhardt R
Cell communication and signaling : CCS 2014 Feb 18;12:11
Cell communication and signaling : CCS 2014 Feb 18;12:11
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Jurkat 5% SDS PAGE GTX104362 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse ESC 5% SDS PAGE GTX104362 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GLI3 antibody detects GLI3 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract B. 30 ?g Rat2 whole cell lysate/extract5% SDS-PAGEGLI3 antibody (GTX104362) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GLI3(GTX104362) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GLI3 antibody detects GLI3 protein at nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GLI3 antibody (GTX104362) diluted at 1:250.