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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [29]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- AF3690 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse GLI-3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse GLI-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) GLI-1 and rhGLI-2 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10071- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The ciliary protein RPGRIP1L governs autophagy independently of its proteasome-regulating function at the ciliary base in mouse embryonic fibroblasts.
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms.
Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.
Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets.
Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.
Suppressor of Fused restraint of Hedgehog activity level is critical for osteogenic proliferation and differentiation during calvarial bone development.
Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation.
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-β2 nuclear import system.
Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling.
Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog.
Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline.
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9.
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number.
T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor.
Sufu and Kif7 in limb patterning and development.
Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis.
GATA6 is a crucial regulator of Shh in the limb bud.
A switch from low to high Shh activity regulates establishment of limb progenitors and signaling centers.
Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis.
Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2).
The hedgehog system machinery controls transforming growth factor-β-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Struchtrup A, Wiegering A, Stork B, Rüther U, Gerhardt C
Autophagy 2018;14(4):567-583
Autophagy 2018;14(4):567-583
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms.
Rosengren T, Larsen LJ, Pedersen LB, Christensen ST, Møller LB
Cellular and molecular life sciences : CMLS 2018 Jul;75(14):2663-2680
Cellular and molecular life sciences : CMLS 2018 Jul;75(14):2663-2680
Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.
He K, Ma X, Xu T, Li Y, Hodge A, Zhang Q, Torline J, Huang Y, Zhao J, Ling K, Hu J
Nature communications 2018 Aug 17;9(1):3310
Nature communications 2018 Aug 17;9(1):3310
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.
Hong SR, Wang CL, Huang YS, Chang YC, Chang YC, Pusapati GV, Lin CY, Hsu N, Cheng HC, Chiang YC, Huang WE, Shaner NC, Rohatgi R, Inoue T, Lin YC
Nature communications 2018 Apr 30;9(1):1732
Nature communications 2018 Apr 30;9(1):1732
Regulation of Calvarial Osteogenesis by Concomitant De-repression of GLI3 and Activation of IHH Targets.
Veistinen LK, Mustonen T, Hasan MR, Takatalo M, Kobayashi Y, Kesper DA, Vortkamp A, Rice DP
Frontiers in physiology 2017;8:1036
Frontiers in physiology 2017;8:1036
Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to hedgehog-mediated osteogenic induction.
Hasegawa D, Ochiai-Shino H, Onodera S, Nakamura T, Saito A, Onda T, Watanabe K, Nishimura K, Ohtaka M, Nakanishi M, Kosaki K, Yamaguchi A, Shibahara T, Azuma T
PloS one 2017;12(10):e0186879
PloS one 2017;12(10):e0186879
Suppressor of Fused restraint of Hedgehog activity level is critical for osteogenic proliferation and differentiation during calvarial bone development.
Li J, Cui Y, Xu J, Wang Q, Yang X, Li Y, Zhang X, Qiu M, Zhang Z, Zhang Z
The Journal of biological chemistry 2017 Sep 22;292(38):15814-15825
The Journal of biological chemistry 2017 Sep 22;292(38):15814-15825
Sonic Hedgehog Activates Phospholipase A2 to Enhance Smoothened Ciliary Translocation.
Arensdorf AM, Dillard ME, Menke JM, Frank MW, Rock CO, Ogden SK
Cell reports 2017 Jun 6;19(10):2074-2087
Cell reports 2017 Jun 6;19(10):2074-2087
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling.
Guen VJ, Chavarria TE, Kröger C, Ye X, Weinberg RA, Lees JA
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 5;114(49):E10532-E10539
Proceedings of the National Academy of Sciences of the United States of America 2017 Dec 5;114(49):E10532-E10539
Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord.
Cai H, Liu A
Developmental biology 2017 Dec 1;432(1):72-85
Developmental biology 2017 Dec 1;432(1):72-85
Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-β2 nuclear import system.
Han Y, Xiong Y, Shi X, Wu J, Zhao Y, Jiang J
PLoS biology 2017 Aug;15(8):e2002063
PLoS biology 2017 Aug;15(8):e2002063
Cholesterol activates the G-protein coupled receptor Smoothened to promote Hedgehog signaling.
Luchetti G, Sircar R, Kong JH, Nachtergaele S, Sagner A, Byrne EF, Covey DF, Siebold C, Rohatgi R
eLife 2016 Oct 5;5
eLife 2016 Oct 5;5
Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog.
Tukachinsky H, Petrov K, Watanabe M, Salic A
Proceedings of the National Academy of Sciences of the United States of America 2016 Oct 4;113(40):E5866-E5875
Proceedings of the National Academy of Sciences of the United States of America 2016 Oct 4;113(40):E5866-E5875
Craniofacial Ciliopathies Reveal Specific Requirements for GLI Proteins during Development of the Facial Midline.
Chang CF, Chang YT, Millington G, Brugmann SA
PLoS genetics 2016 Nov;12(11):e1006351
PLoS genetics 2016 Nov;12(11):e1006351
Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9.
Makino S, Fukumura R, Gondo Y
Scientific reports 2016 Dec 21;6:39608
Scientific reports 2016 Dec 21;6:39608
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling.
Cai H, Liu A
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):14751-14756
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 20;113(51):14751-14756
T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number.
Emechebe U, Kumar P P, Rozenberg JM, Moore B, Firment A, Mirshahi T, Moon AM
eLife 2016 Apr 5;5
eLife 2016 Apr 5;5
T396I mutation of mouse Sufu reduces the stability and activity of Gli3 repressor.
Makino S, Zhulyn O, Mo R, Puviindran V, Zhang X, Murata T, Fukumura R, Ishitsuka Y, Kotaki H, Matsumaru D, Ishii S, Hui CC, Gondo Y
PloS one 2015;10(3):e0119455
PloS one 2015;10(3):e0119455
Sufu and Kif7 in limb patterning and development.
Zhulyn O, Hui CC
Developmental dynamics : an official publication of the American Association of Anatomists 2015 Mar;244(3):468-78
Developmental dynamics : an official publication of the American Association of Anatomists 2015 Mar;244(3):468-78
Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
Chong YC, Mann RK, Zhao C, Kato M, Beachy PA
Genes & development 2015 Feb 1;29(3):262-76
Genes & development 2015 Feb 1;29(3):262-76
Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
Liu YP, Tsai IC, Morleo M, Oh EC, Leitch CC, Massa F, Lee BH, Parker DS, Finley D, Zaghloul NA, Franco B, Katsanis N
The Journal of clinical investigation 2014 May;124(5):2059-70
The Journal of clinical investigation 2014 May;124(5):2059-70
Murine Joubert syndrome reveals Hedgehog signaling defects as a potential therapeutic target for nephronophthisis.
Hynes AM, Giles RH, Srivastava S, Eley L, Whitehead J, Danilenko M, Raman S, Slaats GG, Colville JG, Ajzenberg H, Kroes HY, Thelwall PE, Simmons NL, Miles CG, Sayer JA
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 8;111(27):9893-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 8;111(27):9893-8
GATA6 is a crucial regulator of Shh in the limb bud.
Kozhemyakina E, Ionescu A, Lassar AB
PLoS genetics 2014 Jan;10(1):e1004072
PLoS genetics 2014 Jan;10(1):e1004072
A switch from low to high Shh activity regulates establishment of limb progenitors and signaling centers.
Zhulyn O, Li D, Deimling S, Vakili NA, Mo R, Puviindran V, Chen MH, Chuang PT, Hopyan S, Hui CC
Developmental cell 2014 Apr 28;29(2):241-9
Developmental cell 2014 Apr 28;29(2):241-9
Gli3 regulation of myogenesis is necessary for ischemia-induced angiogenesis.
Renault MA, Vandierdonck S, Chapouly C, Yu Y, Qin G, Metras A, Couffinhal T, Losordo DW, Yao Q, Reynaud A, Jaspard-Vinassa B, Belloc I, Desgranges C, Gadeau AP
Circulation research 2013 Oct 25;113(10):1148-58
Circulation research 2013 Oct 25;113(10):1148-58
Regulation and function of immunosuppressive molecule human leukocyte antigen G5 in human bone tissue.
Deschaseaux F, Gaillard J, Langonné A, Chauveau C, Naji A, Bouacida A, Rosset P, Heymann D, De Pinieux G, Rouas-Freiss N, Sensébé L
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2977-87
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2013 Aug;27(8):2977-87
Prevention of premature fusion of calvarial suture in GLI-Kruppel family member 3 (Gli3)-deficient mice by removing one allele of Runt-related transcription factor 2 (Runx2).
Tanimoto Y, Veistinen L, Alakurtti K, Takatalo M, Rice DP
The Journal of biological chemistry 2012 Jun 15;287(25):21429-38
The Journal of biological chemistry 2012 Jun 15;287(25):21429-38
The hedgehog system machinery controls transforming growth factor-β-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wémeau-Stervinou L, Fabre A, Mal H, Lesèche G, Dehoux M, Soler P, Crestani B, Mailleux AA
The American journal of pathology 2012 Dec;181(6):2126-37
The American journal of pathology 2012 Dec;181(6):2126-37
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
Genes & development 2010 Apr 1;24(7):670-82
Genes & development 2010 Apr 1;24(7):670-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse GLI-3 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI-3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GLI-3 in HeLa Human Cell Line. GLI-3 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GLI-3-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin for 30 minutes was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. GLI-3/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse GLI-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The gli-1 promoter was detected by standard PCR.
