697202

antibody from BioLegend

Targeting: KRT17 PCHC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

697202

Provider

BioLegend

Proper citation

BioLegend Cat#697202, RRID:AB_2687136

Product name

Purified anti-Cytokeratin 17

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16131A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total cell lysates (15µg protein) from A549, A431 and NIH3T3 cells were resolved by 4-20% Tris-glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.5µg/mL purified anti- Cytokeratin 17 (clone W16131A) antibody, competitor’s antibody used at manufactures recommended concentration (upper) or loading control anti-GAPDH (poly6314) antibody (lower). Proteins were visualized by chemiluminescence detection using a goat anti-rat-IgG secondary antibody conjugated to HRP for anti- Cytokeratin 17 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody and anti-GAPDH antibody. Lane M: Molecular Weight ladder.

Conjugate

Unconjugated