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Learn18502-1-AP
antibody from Invitrogen Antibodies
Targeting: KRT17
PCHC1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 18502-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 17 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MTTSIRQFT SSSSIKGSSG LGGGSSRTSC RLSGGLGAGS CRLGSAGGLG STLGGSSYSS CYSFGSGGGY GSSFGGVDGL LAGGEKATMQ NLNDRLASYL DKVRALEEAN TELEVKIRDW YQRQAPGPAR DYSQYYRTIE ELQNKILTAT VDNANILLQI DNARLAADDF RTKFETEQAL RLSVEADING LRRVLDELTL ARADLEMQIE NLKEELAYLK KNHEEEMNAL RGQVGGEINV EMDAAPGVDL SRILNEMRDQ YEKMAEKNRK DAEDWFFSKT EELNREVATN SELVQSGKSE ISELRRTMQA LEIELQSQLS MKASLEGNLA ETENRYCVQL SQIQGLIGSV EEQLAQLRCE MEQQNQEYKI LLDVKTRLEQ EIATYRRLLE GEDAHLTQYK KEPVTTRQVR TIVEEVQDGK VISSREQVHQ TTR (1-432 aa encoded by BC011901)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human pancreas cancer using 18502-1-AP (Cytokeratin 17 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin tissue slide using 18502-1-AP (Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP ( Cytokeratin 17 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 18502-1-AP ( Cytokeratin 17 antibody at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Cytokeratin 17 antibody (18502-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Cytokeratin 17 (IP:18502-1-AP, 4ug; Detection:18502-1-AP 1:600) with HeLa cells lysate 1600ug.
