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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA000452 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000452, RRID:AB_1079177
- Product name
- Anti-KRT17
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
ARDYSQYYRTIEELQNKILTATVDNANILLQIDNA
RLAADDFRTKFETEQALRLSVEADINGLRRVLDEL
TLARADLEMQIENLKEELAYLKKNHEEEMNALRGQ
VGGEINVEMDAAPGVDLSRI- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
Submitted references Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression.
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
Kopecki Z, Yang GN, Jackson JE, Melville EL, Calley MP, Murrell DF, Darby IA, O'Toole EA, Samuel MS, Cowin AJ
Oncotarget 2015 Nov 3;6(34):36426-40
Oncotarget 2015 Nov 3;6(34):36426-40
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
Kiflemariam S, Andersson S, Asplund A, Pontén F, Sjöblom T
PloS one 2012;7(3):e32927
PloS one 2012;7(3):e32927
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Western blot analysis in human cell lines HeLa and PC-3 using Anti-KRT17 antibody. Corresponding KRT17 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
- Sample type
- HUMAN
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human skin shows distinct positivity in keratinocytes.
- Sample type
- HUMAN
