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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG51816 - Provider product page
- Provider
- Arigo
- Product name
- anti-HDAC4/HDAC5/HDAC9 phospho (Ser246/259/220) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S(p)-EP) derived from Human HDAC4/HDAC5/HDAC9.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells untreated or treated with EGF stained with anti-HDAC4/HDAC5/HDAC9 (phospho Ser246/259/220) antibody ARG51816.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: extracts from 293 cells and HeLa cells treated with serum stained with anti-HDAC4/HDAC5/HDAC9 (phospho Ser246/259/220) antibody ARG51816.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-HDAC4/HDAC5/HDAC9 (phospho Ser246/259/220) antibody ARG51816 (left) or the same antibody preincubated with blocking peptide (right).
