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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- F41192 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HDAC9 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 2-32 from the human protein was used as the immunogen for this HDAC9 antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the HDAC9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: HDAC9 antibody NSJ# F41192 and NSJ# F41193 were tested by western blot and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Both antibodies detect the protein in transfected cells but not non-transfected.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- WB: Immunoblots for MITR (HDAC9), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman, Upstate Medical University, New York.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescence staining of MITR (HDAC9) for a compartmentalization study in undifferentiated transfected C2C12 myoblasts. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IP: HDAC9 antibody NSJ# F41192 and NSJ# F41193 can both immunoprecipitate the protein from HeLa-HDAC9 tranfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute)
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma stained with HDAC9 antibody
