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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN950177 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L) (C-Term), (AA 263-291) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 263-291aa) of human 11-beta-HSD3 / HSD11B1L at C-term.
- Description
- Purified through a protein A column, followed by peptide affinity purification
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 263-291
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Isolation and characterization of novel human short-chain dehydrogenase/reductase SCDR10B which is highly expressed in the brain and acts as hydroxysteroid dehydrogenase.
Crystal structure of human ABAD/HSD10 with a bound inhibitor: implications for design of Alzheimer's disease therapeutics.
Huang C, Wan B, Gao B, Hexige S, Yu L
Acta biochimica Polonica 2009;56(2):279-89
Acta biochimica Polonica 2009;56(2):279-89
Crystal structure of human ABAD/HSD10 with a bound inhibitor: implications for design of Alzheimer's disease therapeutics.
Kissinger CR, Rejto PA, Pelletier LA, Thomson JA, Showalter RE, Abreo MA, Agree CS, Margosiak S, Meng JJ, Aust RM, Vanderpool D, Li B, Tempczyk-Russell A, Villafranca JE
Journal of molecular biology 2004 Sep 17;342(3):943-52
Journal of molecular biology 2004 Sep 17;342(3):943-52
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HSD11B1L antibody Cat.-No.AP52106PU-N Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HSD11B1L antibody detected the HSD11B1L protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of HSD11B1L antibody Cat.-No. AP52106PU-N) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- HSD11B1L antibody Cat.-No. AP52106PU-N: immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
