Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-35315 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Transthyretin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with non-human primate based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Transthyretin amyloid fibrils alter primary fibroblast structure, function, and inflammatory gene expression.
Dittloff KT, Iezzi A, Zhong JX, Mohindra P, Desai TA, Russell B
American journal of physiology. Heart and circulatory physiology 2021 Jul 1;321(1):H149-H160
American journal of physiology. Heart and circulatory physiology 2021 Jul 1;321(1):H149-H160
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TTR in human blood plasma tissue lysates (35 µg/lane) using a TTR polyclonal antibody (Product # PA5-35315).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TTR in formalin-fixed, paraffin-embedded human hepatocarcinoma using a TTR polyclonal antibody (Product # PA5-35315) followed by peroxidase conjugation of the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TTR in human pancreas tissue using a TTR polyclonal antibody (Product # PA5-35315) followed by detection using a fluorescent conjugated secondary antibody (green) at a dilution of 1:400. Nuclei were stained with Dapi (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of TTR in A375 cells (right) compared to a negative control (left) using a TTR polyclonal antibody (Product # PA5-35315) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.