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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- AF3505 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD177 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CD177 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAH29167- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation.
Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, Kettritz R
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
The Journal of biological chemistry 2011 Mar 4;286(9):7070-81
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CD177 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Goat Anti-Human CD177 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3505, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
