Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM31111PU-N - Provider product page
- Provider
- OriGene
- Product name
- CD177 mouse monoclonal antibody, clone 4C4, Purified
- Antibody type
- Monoclonal
- Description
- CD177 mouse monoclonal antibody, clone 4C4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CD177
- Isotype
- IgG
- Antibody clone number
- 4C4
- Vial size
- 50 µg
- Concentration
- 0.5 mg/ml
Submitted references Cholemic Nephropathy Causes Acute Kidney Injury and Is Accompanied by Loss of Aquaporin 2 in Collecting Ducts.
Bräsen JH, Mederacke YS, Schmitz J, Diahovets K, Khalifa A, Hartleben B, Person F, Wiech T, Steenbergen E, Großhennig A, Manns MP, Schmitt R, Mederacke I
Hepatology (Baltimore, Md.) 2019 May;69(5):2107-2119
Hepatology (Baltimore, Md.) 2019 May;69(5):2107-2119
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis of CD177 expression in transfected 293T cell line by CD177 monoclonal antibody, clone 4C4.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CD177 is approximately 3ng/ml as a capture antibody.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD177 transfected lysate using anti-CD177 monoclonal antibody and Protein A Magnetic Bead.
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Validation comment
- IHC