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LearnNBP1-90023
antibody from Novus Biologicals
Targeting: MFGE8
BA46, EDIL1, hP47, HsT19888, MFG-E8, OAcGD3S, SED1, SPAG10
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- NBP1-90023 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90023, RRID:AB_11011912
- Product name
- Rabbit Polyclonal MFG-E8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Milk Fat Globule 1 membrane protein antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Stadler C, Rexhepaj E, Singan VR, Murphy RF, Pepperkok R, Uhlén M, Simpson JC, Lundberg E
Nature methods 2013 Apr;10(4):315-23
Nature methods 2013 Apr;10(4):315-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MFG-E8 Antibody [NBP1-90023] - Analysis in human cell lines SK-MEL-30 and MCF-7 using anti-MFGE8 antibody. Corresponding MFGE8 RNA-seq data are presented for the same cell lines. Loading control: anti-PFN1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MFG-E8 Antibody [NBP1-90023] - Analysis in control (vector only transfected HEK293T lysate) and MFGE8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human placenta shows cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining in human heart muscle and liver tissues.. Corresponding MFGE8 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human heart muscle shows moderate to strong cytoplasmic positivity in intercalated discs.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human kidney shows moderate to strong cytoplasmic positivity in aortic media.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MFG-E8 Antibody [NBP1-90023] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
