AF-268-NA

antibody from R&D Systems

Targeting: NTF4 GLC1O, NT-4/5, NTF5

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-268-NA

Provider

R&D Systems

Product name

Human NT-4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human NT-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) NT-3 and rhBDNF is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P34130


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Brain-derived neurotrophic factor and neurotrophin-4/5 are expressed in breast cancer and can be targeted to inhibit tumor cell survival.

Vanhecke E, Adriaenssens E, Verbeke S, Meignan S, Germain E, Berteaux N, Nurcombe V, Le Bourhis X, Hondermarck H
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Apr 1;17(7):1741-52

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by NT-4 and Neutralization by Human NT-4 Antibody. Recombinant Human NT-4 (Catalog # 268-N4) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human NT-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-268-NA). The ND₅₀ is typically 0.4-2 µg/mL.