MA5-34841

antibody from Invitrogen Antibodies

Targeting: MARK3 CTAK1, KP78, PAR-1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34841

Provider

Invitrogen Antibodies

Product name

MARK3 Recombinant Rabbit Monoclonal Antibody (JE44-84)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: Rat brain tissue lysate, A431, 293, MCF-7, SH-SY-5Y, SiHa, human appendix tissue, human breast cancer tissue, mouse small intestine tissue.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JE44-84

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MARK3 in Positive control: Lane 1: rat brain tissue lysate, Lane 2: A431 cell lysate, Lane 3: 293 cell lysate. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with MARK3 monoclonal antibody (Product # MA5-34841), at a dilution of 1:500 (5% BSA, 2 hours), followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MARK3 in MCF-7 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MARK3 in SiHa cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MARK3 in SH-SY-5Y cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MARK3 in paraffin-embedded mouse small intestine tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MARK3 in paraffin-embedded human breast cancer tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MARK3 in paraffin-embedded human appendix tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:100 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of MARK3 in MCF-7 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with MARK3 monoclonal antibody (Product # MA5-34841) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (30 min) with a dilution of 1:500.