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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX121449 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX121449, RRID:AB_10731459
- Product name
- ELP2 antibody [N3C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ug ) A: HeLa nucleus 7.5% SDS PAGE GTX121449 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using ELP2(GTX121449) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELP2?antibody immunoprecipitates ELP2?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of ELP2?protein by 2 £gg of ELP2?antibody (GTX121449) 7.5% SDS-PAGE The immunoprecipitated ELP2?protein was detected by ELP2?antibody (GTX121449) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ELP2(GTX121449) antibody at 1:250 dilution.
