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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324752 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ELP2 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ELP2 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ELP2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ELP2 antibody (C-term) (Cat. #TA324752) in NCI-H460 cell line lysates (35ug/lane). ELP2 (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with ELP2 (C-term) antibody. SY5Y cells were incubated with TA324752 ELP2 (C-term) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the ELP2 immunosignal mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000134759).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H292 cells using ELP2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
