LS-C742096

antibody from LSBio

Targeting: RPL17 L17, rpL23

Western blot (Enhanced validation)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C742096

Provider

LSBio

Product name

RPL17 / Ribosomal Protein L17 Antibody (clone 15B3F5) LS-C742096

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

15B3F5

Storage

Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Human RPL17 recombinant protein. Lane 1: 50 ng Human RPL17 recombinant protein Lane 2: 25 ng Human RPL17 recombinant protein Lane 3: 10 ng Human RPL17 recombinant protein Lane 4: 50 ng Human RPL17 recombinant protein Lane 5: 25 ng Human RPL17 recombinant protein Lane 6: 10 ng Human RPL17 recombinant protein Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 1 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Hela cell lysates. Lane 1: 50 µg Hela cell Lysate Lane 2: 50 µg Hela cell Lysate Lane 3: 50 µg Hela cell Lysate Lane 4: 50 µg Hela cell Lysate Lane 5: 50 µg Hela cell Lysate Lane 6: 50 µg Hela cell Lysate Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 5 µg/ml, 1 µg/ml, 0.5 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Hela cell lysates. Lane 1: 100 µg Hela cell Lysate Lane 2: 50 µg Hela cell Lysate Lane 3: 10 µg Hela cell Lysate Lane 4: 100 µg Hela cell Lysate Lane 5: 50 µg Hela cell Lysate Lane 6: 10 µg Hela cell Lysate Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 1 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Human RPL17 recombinant protein. Lane 1: 50 ng Human RPL17 recombinant protein Lane 2: 25 ng Human RPL17 recombinant protein Lane 3: 10 ng Human RPL17 recombinant protein Lane 4: 50 ng Human RPL17 recombinant protein Lane 5: 25 ng Human RPL17 recombinant protein Lane 6: 10 ng Human RPL17 recombinant protein Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 1 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Hela cell lysates. Lane 1: 50 µg Hela cell Lysate Lane 2: 50 µg Hela cell Lysate Lane 3: 50 µg Hela cell Lysate Lane 4: 50 µg Hela cell Lysate Lane 5: 50 µg Hela cell Lysate Lane 6: 50 µg Hela cell Lysate Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 5 µg/ml, 1 µg/ml, 0.5 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Hela cell lysates. Lane 1: 100 µg Hela cell Lysate Lane 2: 50 µg Hela cell Lysate Lane 3: 10 µg Hela cell Lysate Lane 4: 100 µg Hela cell Lysate Lane 5: 50 µg Hela cell Lysate Lane 6: 10 µg Hela cell Lysate Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 1 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Independent Western Blot validation of Human RPL17 Antibody (15B3F5) and Human RPL17 Antibody (6H11E8) with Hela cell lysates. Lane 1: 100 µg Hela cell Lysate Lane 2: 50 µg Hela cell Lysate Lane 3: 10 µg Hela cell Lysate Lane 4: 100 µg Hela cell Lysate Lane 5: 50 µg Hela cell Lysate Lane 6: 10 µg Hela cell Lysate Primary Antibody: Lane 1~3: Human RPL17 Antibody (6H11E8) 1 µg/ml Lane 4~6: Human RPL17 Antibody (15B3F5) (H&L) [IRDye8°°] (Licor,926-32211)

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Standard curve of RPL17 Sandwich ELISA. The RPL17 Sandwich ELISA assay is developed by using Human RPL17 Antibody (15B3F5) and Biotin conjugated Human RPL17 Antibody (6H11E8) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ELISA binding of Human RPL17 Antibody (15B3F5) with Human RPL17 recombinant protein. Coating antigen: RPL17, 1 µg/ml. RPL17 antibody dilution start from 1000 ng/ml, EC50= 9.728 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Standard curve of RPL17 Sandwich ELISA. The RPL17 Sandwich ELISA assay is developed by using Human RPL17 Antibody (15B3F5) and Biotin conjugated Human RPL17 Antibody (6H11E8) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of Human RPL17 Antibody (15B3F5) with Human RPL17 recombinant protein. Coating antigen: RPL17, 1 µg/ml. RPL17 antibody dilution start from 1000 ng/ml, EC50= 9.728 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Standard curve of RPL17 Sandwich ELISA. The RPL17 Sandwich ELISA assay is developed by using Human RPL17 Antibody (15B3F5) and Biotin conjugated Human RPL17 Antibody (6H11E8) as capture and detect antibody, respectively. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of Human RPL17 Antibody (15B3F5) with Human RPL17 recombinant protein. Coating antigen: RPL17, 1 µg/ml. RPL17 antibody dilution start from 1000 ng/ml, EC50= 9.728 ng/ml.