AP16577b

antibody from Abgent

Targeting: ATF3

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP16577b

Provider

Abgent

Proper citation

Abgent Cat#AP16577b, RRID:AB_11138390

Product name

ATF3 Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.3 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ATF3 Antibody (C-term) (Cat. #AP16577b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ATF3 antibody detected the ATF3 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ATF3 (arrow) using rabbit polyclonal ATF3 Antibody (C-term) (Cat. #AP16577b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.

Primary Ab dilution

1:1000

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

ATF3 Antibody (C-term) (Cat. #AP16577b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50