Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32433 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD36 Recombinant Rabbit Monoclonal Antibody (JJ2005)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JJ2005
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD36 in human heart (1) and mouse heart (2) tissue lysates using a CD36 Monoclonal antibody (Product # MA5-32433) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-CD36 Recombinant Rabbit Monoclonal Antibody (JJ2005) (Product # MA5-32433) and an 88 kDa band corresponding to CD36 was observed. Expression was upregulated in THP-1 differentiated cells using PMA and the expression was reduced in Jurkat when compared to THP-1. Relative expression was seen in adipose, brown adipose with higher expression when compared to other tissues. Membrane enriched extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with PMA (80 nm, 16 hrs) (Lane 2), Jurkat (Lane 3), RAW 264.7 (Lane 4), Hep G2 (Lane 5), K-562 (Lane 6) in Fig. a; tissue extracts of Mouse Adipose (lane 1), Mouse Brown Adipose (Lane 2), Rat Adipose (Lane 3), Rat Brown Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6), Mouse Kidney (Lane 7), Mouse Liver (Lane 8), Rat Testis (Lane 9), Mouse Cerebellum (Lane 10), Mouse Colon (Lane 11) in Fig. b, were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1,000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6,000 dilution) using the iBright FL 1000 (Product # A32752).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD36 in SH-SY5Y cells using a CD36 Monoclonal antibody (Product # MA5-32433) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of CD36 in SH-SY5Y cells using a CD36 Monoclonal antibody (Product # MA5-32433) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Platelet glycoprotein 4 was performed using 70% confluent log phase THP-1 and THP-1 cells differentiated using PMA (80 nm for 16hrs) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD36 Recombinant Rabbit Monoclonal Antibody (JJ2005) (Product # MA5-32433) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane localization. Panel e represents control THP-1 cells with lower expression levels. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CD36 of paraffin-embedded Human spleen tissue using a CD36 Monoclonal antibody (Product #MA5-32433). Counter stained with hematoxylin..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of CD36 in THP-1 cells using a CD36 Monoclonal Antibody (Product # MA5-32433) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody..