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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-29589 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD36 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, Mouse brown adipose. Predicted reactivity: Mouse (83%), Rat (85%), Dog (80%), Pig (82%), Rhesus Monkey (92%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.71 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Sample (50 µg of whole cell lysate). Lane A: Mouse brown adipose . 7.5% SDS PAGE. CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2 . 7.5% SDS PAGE. CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD36 Polyclonal Antibody (Product # PA5-29589). Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 µg/mL gentamicin, 1 nM L-glutamin, 500 µM IBMX, 1 µM dexamethasone, 2 µM rosiglitazone and 1 µg/mL insulin.).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD36 Polyclonal Antibody detects CD36 protein at cytosol and weak membrane on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. CD36 Polyclonal Antibody (Product # PA5-29589) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD36 Polyclonal Antibody detects CD36 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. CD36 stained by CD36 Polyclonal Antibody (Product # PA5-29589) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
