- 13-7000 - Invitrogen Antibodies STAT3 antibody

Submitted references Opposing roles of STAT1 and STAT3 in IL-21 function in CD4+ T cells.

Wan CK, Andraski AB, Spolski R, Li P, Kazemian M, Oh J, Samsel L, Swanson PA 2nd, McGavern DB, Sampaio EP, Freeman AF, Milner JD, Holland SM, Leonard WJ
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 28;112(30):9394-9

IL-21-mediated non-canonical pathway for IL-1β production in conventional dendritic cells.

Wan CK, Li P, Spolski R, Oh J, Andraski AB, Du N, Yu ZX, Dillon CP, Green DR, Leonard WJ
Nature communications 2015 Aug 13;6:7988

Dysregulated STAT1-SOCS1 control of JAK2 promotes mammary luminal progenitor cell survival and drives ERα(+) tumorigenesis.

Chan SR, Rickert CG, Vermi W, Sheehan KC, Arthur C, Allen JA, White JM, Archambault J, Lonardi S, McDevitt TM, Bhattacharya D, Lorenzi MV, Allred DC, Schreiber RD
Cell death and differentiation 2014 Feb;21(2):234-46

BLIMP-1 and STAT3 counterregulate microRNA-21 during plasma cell differentiation.

Barnes NA, Stephenson S, Cocco M, Tooze RM, Doody GM
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 1;189(1):253-60

Src family kinases are important negative regulators of G-CSF-dependent granulopoiesis.

Mermel CH, McLemore ML, Liu F, Pereira S, Woloszynek J, Lowell CA, Link DC
Blood 2006 Oct 15;108(8):2562-8

Clinical and molecular studies of the effect of imatinib on advanced aggressive fibromatosis (desmoid tumor).

Heinrich MC, McArthur GA, Demetri GD, Joensuu H, Bono P, Herrmann R, Hirte H, Cresta S, Koslin DB, Corless CL, Dirnhofer S, van Oosterom AT, Nikolova Z, Dimitrijevic S, Fletcher JA
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2006 Mar 1;24(7):1195-203

Ablation of oncogenic ALK is a viable therapeutic approach for anaplastic large-cell lymphomas.

Piva R, Chiarle R, Manazza AD, Taulli R, Simmons W, Ambrogio C, D'Escamard V, Pellegrino E, Ponzetto C, Palestro G, Inghirami G
Blood 2006 Jan 15;107(2):689-97

PI3K-AKT pathway negatively controls EGFR-dependent DNA-binding activity of Stat3 in glioblastoma multiforme cells.

Ghosh MK, Sharma P, Harbor PC, Rahaman SO, Haque SJ
Oncogene 2005 Nov 10;24(49):7290-300

Malignant transformation but not normal cell growth depends on signal transducer and activator of transcription 3.

Schlessinger K, Levy DE
Cancer research 2005 Jul 1;65(13):5828-34

Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.

Silver DL, Naora H, Liu J, Cheng W, Montell DJ
Cancer research 2004 May 15;64(10):3550-8

Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).

Duensing A, Medeiros F, McConarty B, Joseph NE, Panigrahy D, Singer S, Fletcher CD, Demetri GD, Fletcher JA
Oncogene 2004 May 13;23(22):3999-4006

Constitutive activation of Jak/STAT proteins in Epstein-Barr virus-infected B-cell lines from patients with posttransplant lymphoproliferative disorder.

Nepomuceno RR, Snow AL, Robert Beatty P, Krams SM, Martinez OM
Transplantation 2002 Aug 15;74(3):396-402

Cloning and characterization of IL-10-related T cell-derived inducible factor (IL-TIF), a novel cytokine structurally related to IL-10 and inducible by IL-9.

Dumoutier L, Louahed J, Renauld JC
Journal of immunology (Baltimore, Md. : 1950) 2000 Feb 15;164(4):1814-9

Human interleukin-10-related T cell-derived inducible factor: molecular cloning and functional characterization as an hepatocyte-stimulating factor.

Dumoutier L, Van Roost E, Colau D, Renauld JC
Proceedings of the National Academy of Sciences of the United States of America 2000 Aug 29;97(18):10144-9

Distinct roles for STAT1, STAT3, and STAT5 in differentiation gene induction and apoptosis inhibition by interleukin-9.

Demoulin JB, Van Roost E, Stevens M, Groner B, Renauld JC
The Journal of biological chemistry 1999 Sep 3;274(36):25855-61

ErbB-2 kinase is required for constitutive stat 3 activation in malignant human lung epithelial cells.

Fernandes A, Hamburger AW, Gerwin BI
International journal of cancer 1999 Nov 12;83(4):564-70

Essential role of STAT3 for embryonic stem cell pluripotency.

Raz R, Lee CK, Cannizzaro LA, d'Eustachio P, Levy DE
Proceedings of the National Academy of Sciences of the United States of America 1999 Mar 16;96(6):2846-51

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of STAT3 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAT3 Mouse Monoclonal Antibody (Product # 13-7000) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of STAT3 (ST3-5G7) showing staining in the cytoplasm and nucleus of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- STAT3 Monoclonal Antibody (ST3-5G7) (Product # 13-7000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of STAT3 (ST3-5G7) showing staining in the cytoplasm and nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- STAT3 Monoclonal Antibody (ST3-5G7) (Product # 13-7000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of STAT3 (ST3-5G7) showing staining in the cytoplasm and nucleus of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- STAT3 Monoclonal Antibody (ST3-5G7) (Product # 13-7000) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Gel Mobility Shift Assay using STAT3 monoclonal antibody, mouse (Product # 13-7000). Gel mobility shift analysis was performed with a 32P-labeled optimized GAS probe. Nuclear extracts were prepared from STAT3 (lanes 1-3) or STAT1 (lanes 4-5) transfected 293T cells. STAT proteins were phosphorylated by a co-expressed JAK kinase. STAT3 Monoclonal Antibody, Mouse (ST3-5G7) was added to the reactions as indicated above each lane.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: IP-MS enrichment of STAT3 (LFQ intensity): STAT3 was enriched 84-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and STAT3 antibody (Product # 13-7000). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity.

13-7000

Antibody data