690405

antibody from BioLegend

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

690405

Provider

BioLegend

Proper citation

BioLegend Cat#690405, RRID:AB_2716053

Product name

Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A16002B

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and then treated with IL-6 with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-STAT3 Phospho (Tyr705) (Clone A16002B), or B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human IRF1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated