60199-1-Ig

antibody from Proteintech Group

Targeting: STAT3 APRF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

60199-1-Ig

Provider

Proteintech Group

Proper citation

Proteintech Cat#60199-1-Ig, RRID:AB_10913811

Product name

STAT3 antibody

Antibody type

Monoclonal

Description

KD/KO validated STAT3 antibody (Cat. #60199-1-Ig) is a mouse monoclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

3G2D12

Vial size

20ul, 150ul

References

Submitted references

Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC.

Zhou KX, Huang S, Hu LP, Zhang XL, Qin WT, Zhang YL, Yao LL, Yu Y, Zhou YQ, Zhu L, Ji J, Zhang ZG
Journal of immunology research 2021;2021:6694392

---

Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis.

Pei W, Huang X, Ni B, Zhang R, Niu G, You H
Frontiers in pharmacology 2021;12:730312

---

Elevated Serum FGG Levels Prognosticate and Promote the Disease Progression in Prostate Cancer.

Peng HH, Wang JN, Xiao LF, Yan M, Chen SP, Wang L, Yang K
Frontiers in genetics 2021;12:651647

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Propofol inhibited gastric cancer proliferation via the hsa-miR-328-3p/STAT3 pathway.

Bai ZM, Li XF, Yang Y, Yang YF, Lv DR, Tang LL
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2021 Sep;23(9):1866-1873

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Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.

Jiang M, Li X, Zhang J, Lu Y, Shi Y, Zhu C, Liu Y, Qin B, Luo Z, Du Y, Luo L, Peng L, You J
ACS nano 2021 Sep 28;15(9):14522-14534

---

Noncanonical Wnt5a signaling regulates tendon stem/progenitor cells senescence.

Chen M, Li Y, Xiao L, Dai G, Lu P, Rui Y
Stem cell research & therapy 2021 Oct 18;12(1):544

---

Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells.

Zhao S, Wang C, Lu P, Lou Y, Liu H, Wang T, Yang S, Bao Z, Han L, Liang X, Ma C, Gao L
Journal for immunotherapy of cancer 2021 Nov;9(12)

---

Mucin 16 Promotes Colorectal Cancer Development and Progression Through Activation of Janus Kinase 2.

Liu Z, Gu Y, Li X, Zhou L, Cheng X, Jiang H, Huang Y, Zhang Y, Xu T, Yang W, Huang Q
Digestive diseases and sciences 2021 May 12;

---

Uncovering the anti-NSCLC effects and mechanisms of gypenosides by metabolomics and network pharmacology analysis.

Qi YS, Xie JB, Xie P, Duan Y, Ling YQ, Gu YL, Piao XL
Journal of ethnopharmacology 2021 Dec 5;281:114506

---

Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways.

Wang H, Ai J, Shopit A, Niu M, Ahmed N, Tesfaldet T, Tang Z, Li X, Jamalat Y, Chu P, Peng J, Ma X, Qaed E, Han G, Zhang W, Wang J, Tang Z
Cell biology and toxicology 2021 Aug 28;

---

Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.

Zhang J, Zhu S, Tan Q, Cheng D, Dai Q, Yang Z, Zhang L, Li F, Zuo Y, Dai W, Chen L, Gu E, Xu G, Wei Z, Cao Y, Liu X
Theranostics 2020;10(11):4885-4899

---

CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling.

Huang R, Wang S, Wang N, Zheng Y, Zhou J, Yang B, Wang X, Zhang J, Guo L, Wang S, Chen Z, Wang Z, Xiang S
Cell death & disease 2020 Apr 16;11(4):234

---

Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.

Zhang S, Zhou L, Zhang M, Wang Y, Wang M, Du J, Gu W, Kui F, Li J, Geng S, Du G
Frontiers in pharmacology 2019;10:1658

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Alcohol's Dysregulation of Maternal-Fetal IL-6 and p-STAT3 Is a Function of Maternal Iron Status.

Saini N, Helfrich KK, Kwan STC, Huebner SM, Abazi J, Flentke GR, Blohowiak SE, Kling PJ, Smith SM
Alcoholism, clinical and experimental research 2019 Nov;43(11):2332-2343

---

Inhibition of Connexin43 hemichannels with Gap19 protects cerebral ischemia/reperfusion injury via the JAK2/STAT3 pathway in mice.

Chen B, Yang L, Chen J, Chen Y, Zhang L, Wang L, Li X, Li Y, Yu H
Brain research bulletin 2019 Mar;146:124-135

---

Olean-28,13b-olide 2 plays a role in cisplatin-mediated apoptosis and reverses cisplatin resistance in human lung cancer through multiple signaling pathways.

Zhu B, Ren C, Du K, Zhu H, Ai Y, Kang F, Luo Y, Liu W, Wang L, Xu Y, Jiang X, Zhang Y
Biochemical pharmacology 2019 Dec;170:113642

---

Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways.

Cheng Z, Lei Z, Yang P, Si A, Xiang D, Tang X, Guo G, Zhou J, Hüser N
Molecular carcinogenesis 2019 Aug;58(8):1389-1399

---

Postoperative resveratrol administration improves prognosis of rat orthotopic glioblastomas.

Song X, Shu XH, Wu ML, Zheng X, Jia B, Kong QY, Liu J, Li H
BMC cancer 2018 Sep 3;18(1):871

---

CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.

Li M, Gao J, Li D, Yin Y
Cells 2018 Aug 8;7(8)

---

Effect of modified Xiaochaihu decoction‑containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway.

Chen S, Wang Z, Wan S, Huang H, Liang H
Molecular medicine reports 2017 Nov;16(5):7416-7422

---

CD147 promotes Src-dependent activation of Rac1 signaling through STAT3/DOCK8 during the motility of hepatocellular carcinoma cells.

Wang SJ, Cui HY, Liu YM, Zhao P, Zhang Y, Fu ZG, Chen ZN, Jiang JL
Oncotarget 2015 Jan 1;6(1):243-57

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig(STAT3 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The STAT3 antibody from Proteintech is a mouse monoclonal antibody to a fusion protein of human STAT3. This antibody recognizes human,mouse, rat antigen. The STAT3 antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

60199-1-Ig.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 60199-1-Ig(STAT3 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human lung cancer using 60199-1-Ig(STAT3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue