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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- RQ8454 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NTN3 Antibody / Netrin 3
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100 ug
- Storage
- After reconstitution, the NTN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human RT4, 2) U-251, 3) SH-SY5Y, 4) HeLa and 5) rat liver tissue lysate with NTN3 antibody. Predicted molecular weight ~61 kDa but may be observed at higher molecular weights due to glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HeLa cells with NTN3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human colorectal adenocarcinoma tissue with NTN3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
