PAB28497

antibody from Abnova Corporation

Targeting: STAT4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28497

Provider

Abnova Corporation

Product name

STAT4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant STAT4

Antigen sequence

PMHVAVVISNCLREERRILAAANMPVQGPLEKSLQ
SSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDL
QDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEML
NSLDFKRKEALSKMTQIIHETDL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with STAT4 polyclonal antibody at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with STAT4 polyclonal antibody (Cat # PAB28497) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with STAT4 polyclonal antibody (Cat # PAB28497) shows moderate nucelar positivity in subsets of cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)