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- References [13]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [3]
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- Product number
- 71-7900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-STAT4 (Tyr693) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Cell signaling pathways in autosomal-dominant leukodystrophy (ADLD): the intriguing role of the astrocytes.
Obstructive Lymphangitis Precedes Colitis in Murine Norovirus-Infected Stat1-Deficient Mice.
Targeting Focal Adhesion Kinase Suppresses the Malignant Phenotype in Rhabdomyosarcoma Cells.
Focal adhesion kinase and p53 synergistically decrease neuroblastoma cell survival.
FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors.
A novel VIP signaling pathway in T cells cAMP-->protein tyrosine phosphatase (SHP-2?)-->JAK2/STAT4-->Th1 differentiation.
Regulation of signal transducer and activator of transcription signaling by the tyrosine phosphatase PTP-BL.
Role of H1 receptors in histamine-mediated up-regulation of STAT4 phosphorylation.
Favored T helper 1 response in a mouse model of hepatosteatosis is associated with enhanced T cell-mediated hepatitis.
Increased sensitivity to interferon-alpha in psoriatic T cells.
Interleukin-12-induced interferon-gamma production by human peripheral blood T cells is regulated by mammalian target of rapamycin (mTOR).
Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.
STAT4 activation in smokers and patients with chronic obstructive pulmonary disease.
Ratti S, Rusciano I, Mongiorgi S, Owusu Obeng E, Cappellini A, Teti G, Falconi M, Talozzi L, Capellari S, Bartoletti-Stella A, Guaraldi P, Cortelli P, Suh PG, Cocco L, Manzoli L, Ramazzotti G
Cellular and molecular life sciences : CMLS 2021 Mar;78(6):2781-2795
Cellular and molecular life sciences : CMLS 2021 Mar;78(6):2781-2795
Obstructive Lymphangitis Precedes Colitis in Murine Norovirus-Infected Stat1-Deficient Mice.
Seamons A, Treuting PM, Meeker S, Hsu C, Paik J, Brabb T, Escobar SS, Alexander JS, Ericsson AC, Smith JG, Maggio-Price L
The American journal of pathology 2018 Jul;188(7):1536-1554
The American journal of pathology 2018 Jul;188(7):1536-1554
Targeting Focal Adhesion Kinase Suppresses the Malignant Phenotype in Rhabdomyosarcoma Cells.
Waters AM, Stafman LL, Garner EF, Mruthyunjayappa S, Stewart JE, Mroczek-Musulman E, Beierle EA
Translational oncology 2016 Aug;9(4):263-73
Translational oncology 2016 Aug;9(4):263-73
Focal adhesion kinase and p53 synergistically decrease neuroblastoma cell survival.
Gillory LA, Stewart JE, Megison ML, Waters AM, Beierle EA
The Journal of surgical research 2015 Jun 15;196(2):339-49
The Journal of surgical research 2015 Jun 15;196(2):339-49
FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors.
Megison ML, Gillory LA, Stewart JE, Nabers HC, Mrozcek-Musulman E, Beierle EA
Molecular cancer research : MCR 2014 Apr;12(4):514-26
Molecular cancer research : MCR 2014 Apr;12(4):514-26
A novel VIP signaling pathway in T cells cAMP-->protein tyrosine phosphatase (SHP-2?)-->JAK2/STAT4-->Th1 differentiation.
Liu L, Yen JH, Ganea D
Peptides 2007 Sep;28(9):1814-24
Peptides 2007 Sep;28(9):1814-24
Regulation of signal transducer and activator of transcription signaling by the tyrosine phosphatase PTP-BL.
Nakahira M, Tanaka T, Robson BE, Mizgerd JP, Grusby MJ
Immunity 2007 Feb;26(2):163-76
Immunity 2007 Feb;26(2):163-76
Role of H1 receptors in histamine-mediated up-regulation of STAT4 phosphorylation.
Liu Z, Kharmate G, Patterson E, Khan MM
International immunopharmacology 2006 Mar;6(3):485-93
International immunopharmacology 2006 Mar;6(3):485-93
Favored T helper 1 response in a mouse model of hepatosteatosis is associated with enhanced T cell-mediated hepatitis.
Kremer M, Hines IN, Milton RJ, Wheeler MD
Hepatology (Baltimore, Md.) 2006 Jul;44(1):216-27
Hepatology (Baltimore, Md.) 2006 Jul;44(1):216-27
Increased sensitivity to interferon-alpha in psoriatic T cells.
Eriksen KW, Lovato P, Skov L, Krejsgaard T, Kaltoft K, Geisler C, Ødum N
The Journal of investigative dermatology 2005 Nov;125(5):936-44
The Journal of investigative dermatology 2005 Nov;125(5):936-44
Interleukin-12-induced interferon-gamma production by human peripheral blood T cells is regulated by mammalian target of rapamycin (mTOR).
Kusaba H, Ghosh P, Derin R, Buchholz M, Sasaki C, Madara K, Longo DL
The Journal of biological chemistry 2005 Jan 14;280(2):1037-43
The Journal of biological chemistry 2005 Jan 14;280(2):1037-43
Activated signal transducer and activator of transcription (STAT) 3: localization in focal adhesions and function in ovarian cancer cell motility.
Silver DL, Naora H, Liu J, Cheng W, Montell DJ
Cancer research 2004 May 15;64(10):3550-8
Cancer research 2004 May 15;64(10):3550-8
STAT4 activation in smokers and patients with chronic obstructive pulmonary disease.
Di Stefano A, Caramori G, Capelli A, Gnemmi I, Ricciardolo FL, Oates T, Donner CF, Chung KF, Barnes PJ, Adcock IM
The European respiratory journal 2004 Jul;24(1):78-85
The European respiratory journal 2004 Jul;24(1):78-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using Rb anti-phospho-STAT4 (Product # 71-7900) in lysates from double-transfected 293 cells expressing STAT4 and mutant, non-activatable JAK1 (lane 1) or wild-type JAK1 (lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of STAT4 (pY693) was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with STAT4 (pY693) Rabbit polyclonal Antibody (Product # 71-7900) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing nuclear localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Lamin B1 overexpression induces the phosphorylation of inflammation mediators. Following transduction with mock ( GFP ) and Lamin B1 ( ovLMNB1 ) coding vector, primary dermal fibroblasts from one healthy donor were evaluated for NF-kB and Stat4 phosphorylation and compared to wild type cells ( WT ) ( a ). b representative western blot showing NF-kB and Stat4 phosphorylation in fibroblasts from healthy donors ( CTRLs ) and ADLD patients ( ADLDs ). beta-tubulin was used as loading control. c quantitative analysis of Lamin B1 expression, NF-kB and Stat4 phosphorylation in fibroblasts from healthy donors ( CTRLs , n = 6) and ADLD patients ( ADLDs , n = 6). Data are normalized to beta-tubulin and are expressed as average +- SD. ** indicates p < 0.01 vs. control group
