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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunoprecipitation [1]
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- Product number
- PA-ST4 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse STAT4 Antibody
- Antibody type
- Polyclonal
- Description
- Detects human and murine STAT4. Does not cross-react with any of the other STAT proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 uL
- Storage
- Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Submitted references Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
Liao W, Lin JX, Wang L, Li P, Leonard WJ
Nature immunology 2011 Jun;12(6):551-9
Nature immunology 2011 Jun;12(6):551-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Immunoprecipitation of Human STAT4. STAT4 was immunoprecipitated from lysates (1 x 106 - 107 cells) of human peripheral blood lymphocytes (PBL) following incubation with 3 µg Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4) for 1 hour at 2 - 8 ºC. STAT4-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immunoprecipitated STAT4 was detected by Western blot using a 1:2000 dilution of Human/Mouse STAT4 Polyclonal Antibody (Catalog # PA-ST4). View our recommended buffer recipes for immunoprecipitation.
