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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4536 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4536, RRID:AB_1579136
- Product name
- PRKCD polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCD.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Functional analyses of mammalian protein kinase C isozymes in budding yeast and mammalian fibroblasts.
Molecular and biochemical characterization of a recombinant human PKC-delta family member.
Nomoto S, Watanabe Y, Ninomiya-Tsuji J, Yang LX, Nagai Y, Kiuchi K, Hagiwara M, Hidaka H, Matsumoto K, Irie K
Genes to cells : devoted to molecular & cellular mechanisms 1997 Oct;2(10):601-14
Genes to cells : devoted to molecular & cellular mechanisms 1997 Oct;2(10):601-14
Molecular and biochemical characterization of a recombinant human PKC-delta family member.
Aris JP, Basta PV, Holmes WD, Ballas LM, Moomaw C, Rankl NB, Blobel G, Loomis CR, Burns DJ
Biochimica et biophysica acta 1993 Aug 19;1174(2):171-81
Biochimica et biophysica acta 1993 Aug 19;1174(2):171-81
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCD polyclonal antibody (Cat # PAB4536) in placenta lysate . PRKCD delta (Arrow) was detected using purified PRKCD polyclonal antibody (Cat # PAB4536) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCD (arrow) using rabbit PRKCD polyclonal antibody (Cat # PAB4536). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCD polyclonal antibody (Cat # PAB4536) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
