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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005580-M07 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005580-M07, RRID:AB_547301
- Product name
- Mouse Monoclonal PKC delta Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PRKCD - protein kinase C, delta
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PKC delta Antibody (8E12) [H00005580-M07] - Analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M07), clone 8E12. Lane 1: PRKCD transfected lysatE (77.5 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PKC delta Antibody (8E12) [H00005580-M07] - Detection limit for recombinant GST tagged PRKCD is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PKC delta Antibody (8E12) [H00005580-M07] - Analysis of monoclonal antibody to PRKCD on HeLa cell. Antibody concentration 10 ug/ml
