CABT-B9294

antibody from Creative Diagnostics

Targeting: PRKCD

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

CABT-B9294

Provider

Creative Diagnostics

Product name

Mouse anti-Human PKCIJ monoclonal antibody, clone 38/QLDIJ

Antibody type

Monoclonal

Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes, such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. Conventional PKC (cPKC) subfamily members (Ï«, ÉÇ, and ÎÛ isoforms) consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the the C2-like domains of novel PKC (nPKC) subfamily members (Ñ´, š÷, »Ò, and IJ isoforms) are Ca2+-independent. The atypical PKC (aPKC) subfamily members (šø, êò, and Öñ isoforms) lack the C2 domain and are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC members. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCIJ transcripts are expressed in most tissues with the highest levels being found in hematopoietic tissues and cell lines, including T cells and thymocytes. PKCIJ mRNA is readily detectable in skeletal muscle, lung and brain. However, PKCIJ expression is not detected in several human carcinoma cell lines. Abundant expression of this PKC isozyme in hematopoietic cells suggest that it may have a role in growth and differentiation processes of these cells.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG