19132-1-AP

antibody from Invitrogen Antibodies

Targeting: PRKCD

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

19132-1-AP

Provider

Invitrogen Antibodies

Product name

PKC delta Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DMQDNSGTY GKIWEGSSKC NINNFIFHKV LGKGSFGKVL LGELKGRGEY FAIKALKKDV VLIDDDVECT MVEKRVLTLA AENPFLTHLI CTFQTKDHLF FVMEFLNGGD LMYHIQDKGR FELYRATFYA AEIMCGLQFL HSKGIIYRDL KLDNVLLDRD GHIKIADFGM CKENIFGESR ASTFCGTPDY IAPEILQGLK YTFSVDWWSF GVLLYEMLIG QSPFHGDDED ELFESIRVDT PHYPRWITKE SKDILEKLFE REPTKRLGVT GNIKIHPFFK TINWTLLEKR RLEPPFRPKV KSPRDYSNFD QEFLNEKARL SYSDKNLIDS MDQSAFAGFS FVNPKFEHLL ED (326-676 aa encoded by BC043350)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC delta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 19132-1-AP (PKC delta antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 19132-1-AP ( PKC delta antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain tissue slide using 19132-1-AP ( PKC delta antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PKC delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug.