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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- ANT-085-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SLC17A9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 X g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 1,2. Anti-SLC17A9 Antibody (#ANT-085), (1:200).3,4. Anti-SLC17A9 Antibody , preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate: - 1. Anti-SLC17A9 Antibody (#ANT-085), (1:400). 2. Anti-SLC17A9 Antibody , preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 1,2. Anti-SLC17A9 Antibody (#ANT-085), (1:200).3,4. Anti-SLC17A9 Antibody , preincubated with SLC17A9 Blocking Peptide (#BLP-NT085).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of VNUT (SLC17A9) in rat hypothalamus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC17A9 Antibody (#ANT-085), (1:2000), followed by goat Anti-rabbit-AlexaFluor-488. A. VNUT immunoreactivity (green) in the paraventricular nucleus (PVN) of the hypothalamus is detected in neurons (vertical arrows) and axonal processes (horizontal arrows). 3V = third ventricle. B. VNUT staining (green) in the supra-optic nucleus (SON) of the hypothalamus is observed in neurons (vertical arrows) and axonal processes (horizontal arrows). OT = Optic tract. Cell nuclei are stained with DAPI (blue).
