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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- AF3786 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Vav-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Vav-1 in Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P15498- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta)-dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane.
Oakley FD, Smith RL, Engelhardt JF
The Journal of biological chemistry 2009 Nov 27;284(48):33255-64
The Journal of biological chemistry 2009 Nov 27;284(48):33255-64
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Vav-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Vav-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3786) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vav-1 at approximately 110 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
