MAB6085

antibody from Novus Biologicals

Targeting: HS3ST4 3OST4

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6085

Provider

Novus Biologicals

Product name

Mouse Monoclonal Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HS3ST3B1 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.

Biroccio A, Cherfils-Vicini J, Augereau A, Pinte S, Bauwens S, Ye J, Simonet T, Horard B, Jamet K, Cervera L, Mendez-Bermudez A, Poncet D, Grataroli R, de Rodenbeeke CT, Salvati E, Rizzo A, Zizza P, Ricoul M, Cognet C, Kuilman T, Duret H, Lépinasse F, Marvel J, Verhoeyen E, Cosset FL, Peeper D, Smyth MJ, Londoño-Vallejo A, Sabatier L, Picco V, Pages G, Scoazec JY, Stoppacciaro A, Leonetti C, Vivier E, Gilson E
Nature cell biology 2013 Jul;15(7):818-28

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Heparan Sulfate 3-O-Sulfotransferase 4/ HS3ST4 in Human Brain. Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Mouse Anti-Human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB6085) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.