12618-1-AP

antibody from Invitrogen Antibodies

Targeting: CDH13 CDHH

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12618-1-AP

Provider

Invitrogen Antibodies

Product name

Cadherin-13 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TKKEFQATV EEGAVGVIVN LTVEDKDDPA TGAWRAAYTI INGNPGQSFE IHTNPQTNEG MLSVVKPLDY EISAFHTLLI KVENEDPLVP DVSYGPSSTA TVHITVLDVN EGPVFYPDPM MVTRQEDLSV GSVLLTVNAT DPDSLQHQTI RYSVYKDPAG WLNINPINGT VDTTAVLDRE STFVDNSVYT ALFLAIDSGN PPATGTGTLL ITLEDVNDNA PFIYPTVAEV CDDAKNLSVV ILGASDKDLH PNTDPFKFEI HKQAVPDKVW KISKINNTHA LVSLLQNLNK ANYNLPIMVT DSGKPPMTNI TDLRVQVCSC RNSKVDCNAA GALRFSLPSV LLLSLFSLAC L (364-713 aa encoded by BC030653)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L02 cells were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human endometrial cancer using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:100 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were stained with 0.5ug Cadherin-13 antibody (12618-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug.