Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4207 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL17/VCC-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL17/VCC-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse CXCL17 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q6UXB2
- Isotype
- IgG
- Antibody clone number
- 422208
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MiR-4513 mediates the proliferation and apoptosis of oral squamous cell carcinoma cells via targeting CXCL17.
CXCL17 is a major chemotactic factor for lung macrophages.
Xu YX, Sun J, Xiao WL, Liu YS, Yue J, Xue LF, Deng J, Zhi KQ, Wang YL
European review for medical and pharmacological sciences 2019 May;23(9):3821-3828
European review for medical and pharmacological sciences 2019 May;23(9):3821-3828
CXCL17 is a major chemotactic factor for lung macrophages.
Burkhardt AM, Maravillas-Montero JL, Carnevale CD, Vilches-Cisneros N, Flores JP, Hevezi PA, Zlotnik A
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1468-74
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 1;193(3):1468-74
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL17/VCC-1 in A549 Human Cell Line. CXCL17/VCC-1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCL17/VCC-1 in A549 Human Cell Line by Flow Cytometry. A549 human lung carcinoma cell line was stained with Mouse Anti-Human CXCL17/VCC-1 Monoclonal Antibody (Catalog # MAB4207, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.