STJ23493

antibody from St John's Laboratory

Targeting: EGLN2 HIFPH1, PHD1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

STJ23493

Provider

St John's Laboratory

Product name

Anti-EGLN2 antibody (150-250) (STJ23493)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody anti-EGLN2 (150-250) is suitable for use in Western Blot and Immunoprecipitation.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NA


Epitope

NA

Isotype

IgG

Antibody clone number

NA

Vial size

NA

Concentration

NA

Storage

Store in a freezer at-20°C and avoid freeze-thaw cycles.

Handling

NA

Western blot

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2) ) antibody (STJ23493) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

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Chromatin Immunoprecipitation

Supportive validation

Submitted by

St John's Laboratory (provider)

Main image

Experimental details

Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2) ) antibody (STJ23493). Western blot was performed from the immunoprecipitate using Prolyl hydroxylase PHD1 (Prolyl hydroxylase PHD1 (EGLN2) ) antibody (STJ23493) at a dilition of 1:1000.

Sample type

NA

Validation comment

NA

Primary Ab dilution

NA

Other comments

NA

Secondary Ab

NA

Secondary Ab dilution

NA

Protocol

NA

Appendix

NA

Show more