Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- ELISA [1]
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Validation data
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- Product number
- 835104 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#835104, RRID:AB_2632642
- Product name
- Purified anti-β-Amyloid, 1-11
- Antibody type
- Monoclonal
- Description
- Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APPs) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse species of Aβ with differential propensities to fibrillize and deposit in Senile Plaques.According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD). The 770 amino acid form (APP770) has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- NAB 228
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- IHC staining of anti-β-Amyloid, 1-11 antibody (clone NAB 228) on formalin-fixed paraffin-embedded human Alzheimer’s diseased neocortical brain tissue. After antigen retrieval using 70% Formic Acid for 20 minutes, the tissue was incubated with the primary antibody at 10 µg/ml for 24 hours at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
- Conjugate
- Unconjugated