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LearnAF3678
antibody from R&D Systems
Targeting: CADM3
BIgR, FLJ10698, IGSF4B, Necl-1, NECL1, SynCAM3, TSLL1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF3678 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IGSF4B/SynCAM3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IGSF4C is observed and less than 2% cross-reactivity with rhIGSF4, rhIGSF4D, and rhIGSF8 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8N126- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Human Umbilical Cord Mesenchymal Stem Cells Alleviate Myocardial Endothelial-Mesenchymal Transition in a Rat Dilated Cardiomyopathy Model.
Zhang C, Zhou Y, Lai X, Zhou G, Wang H, Feng X, Chen Y, Wu Y, Wang T, Ma L
Transplantation proceedings 2019 Apr;51(3):936-941
Transplantation proceedings 2019 Apr;51(3):936-941
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IGSF4B/SynCAM3 in BG01V Human Embryonic Stem Cells. IGSF4B/SynCAM3 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Goat Anti-Human IGSF4B/SynCAM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3678) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
