Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB7335 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD90/Thy1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD90/Thy1 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD90/Thy1 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P01831- Isotype
- IgG
- Antibody clone number
- 778053
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Establishment of in vitro model of erectile dysfunction for the study of high-glucose-induced angiopathy and neuropathy.
Yin GN, Park SH, Song KM, Limanjaya A, Ghatak K, Minh NN, Ock J, Ryu JK, Suh JK
Andrology 2017 Mar;5(2):327-335
Andrology 2017 Mar;5(2):327-335
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse CD90/Thy1 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD90/Thy1 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD90/Thy1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD90/Thy1 Monoclonal Antibody (Catalog # MAB7335) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
